The basics of microbiology for the water treatment industry

Microbiology is the study of microscopic single-celled organisms such as bacteria, fungi, yeast, viruses, protozoa and algae. In the water treatment industry, the main micro-organisms of concern are bacteria. Algae and fungi are only problematic in certain applications.

Bacterial growth in water systems

Bacteria can use many compounds as a food source. Specific strains of bacteria can metabolise anything from organic material to sulphate and even cyanide. The level of oxygen in a system will also affect the type of bacteria that can grow. Some bacteria require the presence of oxygen for growth (aerobic bacteria) and others will only grow in the complete absence of oxygen (anaerobic bacteria).

The temperature of a particular water system will also determine what bacteria can grow. Most bacteria grow optimally between 15oC to 40oC, but some less common types of bacteria can grow at temperatures as low as 5oC and as high as 60oC.

Problems associated with bacterial growth

There are a number of problems associated with bacterial growth in the water treatment industry. These include:

  • Biofilm formation leading to under deposit corrosion.
  • The breakdown of essential components of water treatment chemical formulations, such as corrosion inhibitors by bacteria.
  • Biofilm formation leading to interference with heat transfer and fluid flow
  • Taste and odour problems in potable (drinking) water.
  • Odour problems, particularly in wastewater treatment applications.
  • The proliferation of Legionella bacteria.
  • The growth of pathogenic (potential disease-causing) bacteria.

In this blog post, we focus on the first three points. We will explore the remaining points in an future article.

Biofilm formation

When the free-swimming bacteria in a system rises to more than 10,000 per ml, the likelihood that a biofilm will form is greatly increased.

The first step in the formation of a biofilm is the adherence of individual bacteria cells to a surface, such as the interior of a pipe. As bacteria reproduce by binary fission (dividing in two), one bacterial cell soon develops into a colony of bacteria on the surface. The bacteria also produce a glue-like substance (exopolysaccharide) which covers the bacterial cells and protects them from the effects of biocides.

Indirect and direct microbiologically induced corrosion (MIC)

For a corrosion inhibitor to work effectively, there has to be contact with the metal surface. A layer of biofilm on the internal surface of a pipe, for example, will prevent the corrosion inhibitor from contacting the metal. Corrosion can, therefore, occur underneath a layer of biofilm.

The fact that bacteria can also consume essential components of corrosion inhibitor formulations, such as nitrite, may lead to corrosion occurring, particularly within closed-circuit systems. Both of these are examples of indirect microbiologically induced corrosion. Certain bacteria, such as Sulphate-reducing bacteria (SRB), will grow in the absence of oxygen (anaerobic growth). An ideal place for the growth of SRB is underneath a biofilm where oxygen is generally unavailable. One of the end products of the metabolism of SRB is hydrogen sulphide, which can be converted into sulphuric acid by sulphide oxidising bacteria. The production of sulphuric acid by these bacteria will cause corrosion of metal surfaces.

Here is an example of Direct MIC on the inner surface of closed circuit pipework:


Biofilm formation leading to interference with heat transfer and fluid flow

Biofilm is the second greatest insulator after silica scale, and a build-up of biofilm on heat transfer surfaces will adversely affect heat transfer efficiency, resulting in increased energy usage. A build-up of biofilm can also block pipework and strainers, restricting fluid flow within a system.

Controlling bacterial levels in water systems

Bacteria levels within water systems can be controlled in two main ways: through the addition of biocides, and with temperature control.


The growth of bacteria can be extremely problematic in many areas of water treatment. It is important to ensure that microbial numbers are under control in systems including drinking water, down water services, process waters, closed circuits, recreational waters and open cooling systems.

Jill Cooper

Jill Cooper

Jill Cooper is Own Label Manager at B&V Chemicals, a leading UK and Export distributor of Water Treatment chemicals for Cooling Water Systems chemicals, Closed Circuit chemicals, Boiler chemicals, Hot and Cold Water System Chemicals, Effluent and Wastewater chemicals. A Microbiologist and Chemist, she has 20+ years production, toll blending and regulatory knowledge.

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